Doctoral Schools

Applied Flow Cytometry

Target group

Members of the Doctoral School of Life Sciences and Medicine

Level

Basic knowledge in flow cytometry

Organizers

- Prof. dr. Evelyne Meyer [] en ing. Kristel Demeyere (lab responsible) - Research group Biochemie, Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary, Universiteit Gent, Salisburylaan 133, 9820 Merelbeke. Tel. 09 264 73 29

- Prof. dr. Bert Devriendt - Research group Immunologie, Department of Virology, Parasitology and Immunology, Faculty of Veterinary, Universiteit Gent, Salisburylaan 133, 9820 Merelbeke. Tel. 09 264 73 43

- Prof. dr. Karine Breckpot [Karine.Breckpot@vub.be] - Laboratory for Molecular and Cellular Therapy, Department of Biomedical Sciences, Vrije Universiteit Brussel

- Prof. dr. Stefaan Verhulst [Stefaan.Verhulst@vub.be] - Scientific Director of Flow Cytometry Core Facility, Vrije Universiteit Brussel

- Prof. dr. Nathalie Cools - Laboratory of Experimental Haematology, Vaccine & Infectious Diseases Institute (VAXINFECTIO), Universiteit Antwerpen, Universitair Ziekenhuis Antwerpen, Wilrijkstraat 10, 2650 Edegem. Tel. 03 821 3584

Programme and venue (3-days course)

Note: Lunch will be provided for you.

Monday 26 September 2022 - 09:30-13:00 / 14:00-16:00

A. Basic Principles of Flow Cytometry (Stefaan Verhulst) and Line up of applications in flow cytometry (Introduction by Karine Breckpot followed by presentation of individual researchers using flow cytometry in a particular application) (morning).


B. An introduction to compensations and different flow cytometers and their specificity (Angelo Willems) (afternoon).

  • Location : Laboratory for Molecular and Cellular Therapy, Department of Biomedical Sciences, Vrije Universiteit Brussel, Laarbeeklaan 103 building E upper floor, meeting room, B-1090 Brussels, Belgium – In case of emergency, contact Karine Breckpot on 0032-2-477 45 66 or 0032-494 25 38 31

 

Tuesday 27 September 2022 - 9:30-12:00 / 13:00- 16:00

A. Detection of selected cytokines in a murine tumor lysate using luminex kit for cytometer bead array analysis.  Practical aspects in the Biochemistry laboratory and explanation of the measurements by the person in charge at the 3-laser flow cytometer with attention to the pitfalls in multiplex bead analysis (morning).

B. Purification of 4 different immune cells (cytotoxic T cells, NK cells, monocytes and pDCs) from the PBMC fraction in the pig. Practical aspects at the Laboratory of Veterinary Immunology, explanation of sorting process by the person responsible for the cell sorter and ‘tip and tricks’ to prepare an efficient sort (afternoon).

 

Wednesday 28 September 2022 - 10:00-12:00 / 13:00-16:00

A. Detection of antigen-specific T cells by intracellular cytokine determination (ICS) (morning).

B. Practical aspects: performing protocol (fix / perm) + antibody staining, followed by flow cytometric analysis with extra attention to pitfalls in ICS (afternoon).

  • Location: UZA, Building 8, Center for Cell Therapy and Regenerative Medicine (CCRG) Universiteit Antwerpen, Drie Eikenstraat 655 - 2650 Edegem. Tel: 03/ 821 35 84. Campus map: here.

In case of emergency contact Nathalie Cools Nathalie.cools@uantwerpen.be , 03/ 821 35 84, 0496/497465

Registration fee

Free of charge for the selected participants of the Doctoral School of Life Sciences and Medicine of Ghent University.

Registration procedure

To register please follow this link: https://eventmanager.ugent.be/AppFlowCyto  In case the course is fully booked, you can ask by e-mail to be added to the waiting list. Please mention your student ID nr.

Please read the cancellation policy: cancellation policy courses

Language

English

Number of participants

Maximum 6

Evaluation criteria (doctoral training programme)

Presence + active participation in all sessions